Diagnosis


How is it Diagnosed? Stool samples are to be collected in C.B. Media & examined under microscope. By culture on TCBS (Thiosulfate – Citrate – Bile salts – Sucrose) media for bacterial growth. Collection and Transport of Stool Samples
  • Collect the stool sample before the patient receives antibiotics.
  • Use a clean cotton tipped swab and introduce well into the rectum. The swab will become moist and may be faecally stained.
  • Alternatively, collect freshly passed liquid stool in a bottle or a cotton tipped swab.
  • Send the sample to the laboratory in a tightly sealed screw capped sterile bottle if the sample can reach the laboratory within two hours.
  • Send the sample to the laboratory in a tightly sealed screw capped sterile bottle with Cary–Blair transport medium (or VR medium or Alkaline Peptone Water) if it will take more than two hours to reach the laboratory.
  • If transport medium is not available, soak strips of blotting paper with liquid stool. Send these to the laboratory in carefully sealed plastic bag to prevent drying.
  • Send the samples using a cold chain.
  • Bottles or plastic bags should be placed in separate plastic bags each to prevent leakage of the potentially contaminated material.
  • Each sample should be labelled. Detailed information should be sent for each sample.